Luciferase is the key enzyme that catalyzes the oxygenation of luciferin, generating energy-rich peroxidic intermediates, emitting a photon of visible light. Inositol 1, 4, 5- trisphosphate (IP3) plays a vital role in the control of a large number of cellular processes.Alteration in its content has been observed in metabolic disorders. Hence, development of high-throughput screening systems to monitor temporal changes of IP3 is essential for screening of new potential therapeutic compounds. Toward a sensitive, rapid and simple method to measuring IP3, we describe the development and application of a luciferasefragment complementation strategy, which converts the ligand-induced conformational changes to light. In order to create a good performance sensor for IP3 monitoring, IP3 binding domain (IP3BD) of IP3R that consist of all the specific binding site of IP3 was selected as a ligand binding domain. The NLuc of firefly luciferase is fused to the Nterminus of IP3BD whiles the CLuc of luciferase is attached to the C-terminus of IP3BD (NLuc-IP3BD-CLuc). A flexible peptide linker (G4S) 2 was inserted between the fragmented luciferase and IP3BD to assist in proper protein folding and luciferase complementation. According to the results, the screening time was very fast and maximum response was obtained up to 11-fold higher than background. Moreover, the designed biosensor was able to monitor release of IP3 upon induction by different inducer like Bradykinin and ATP. The current biosensor not only provides a specific IP3 detector in vitro, but also facilitates to monitoring of the response of IP3 in living organisms.

For a compact K, a necessary condition for C(K) to have the Controlled Separable complementation Property is that K be monolithic. In this paper, we prove that when K contains no copy of [0,ww] and the set of points which admit a countable neighborhood base is a cofinite subset of K, then monolithicity of K is sufficient for C(K) to enjoy the Controlled Separable complementation Property. We also show that, for this type of compacta K, the space C(K) is separably extensible.

Integral and differential of fractional order are important notion that is often used in dealing with Frechet geometry. Based on pseudo-operations given by monotone and continuous function g, we study pseudo-derivative and pseudo-integral of fractional order through this paper.

In this paper, the notion of generalization of pseudo p-closure, denoted by gcl, is introduced and its related properties are investigated. The gcl of subalgebras and pseudo-ideals is discussed. Also, a necessary and sufficient condition for an element to be minimal; and for pseudo BCI-algebra to be nilpotent are given. It is proved that the set of all nilpotent elements of a pseudo BCI-algebra A, denoted by NA, is the least closed pseudo-ideal with the property gcl(NA)=NA. Finally, it is shown that the mentioned notion, as a function, defines a closure operation on pseudo-ideals.